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产品图片 产品编号 品牌 CAS号 产品名称 分子式 购物车
C22-100-04 Perfemiker rHu Her2 | 重组人 Her2/ERBB2 蛋白
Recombinan Human Her2/ERBB2 Protein

Gene Name Synonym:
CD340; ENV; ENVW; ERVWE1; HER-2; HER-2/neu; HER2; HERV-7q;HERV-W-ENV; HERV7Q; HERVW; HERVWENV; MLN 19; MLN19; NEU;NGL; TKR1

纯度:> 90 % as determined by SDS-PAGE
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
表达宿主:HEK293 Cells
种属:Human
预测 :N 端Thr 23
分子量:The recombinant human ErbB2 comprises 641 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, rh ErbB2 migrates as an approximately 100-110 kDa protein in SDS-PAGE under reducing conditions.
生物活性
1. Measured by its binding ability in a functional ELISA.
Immobilized human Erbb2 at 2.5 μg/ml (100 μl/well) can bind Herceptin with a linear range of 1.28-32 ng/ml.
2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.2-1 μg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
3. Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant (His Tag) (Cat: 10004-H08H) bound to Pertuzumab was 0.1 nM.
蛋白构建:
A DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ErbB2 (NP_004439.2) was fused with the polyhistidine tag at the C-terminus.
缓冲液:
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式:
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件:
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶:
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
 

价格库存
A0168 Sigma-Aldrich 抗-小鼠IgG(Fc特异性)-过氧化物酶 山羊抗
Anti-Mouse IgG (Fc Specific)-Peroxidase produced in goat, affinity isolated antibody adsorbed with human IgG

Anti-Mouse IgG (Fc Specific)-Peroxidase produced in goat, affinity isolated antibody adsorbed with human IgG

抗-小鼠IgG(Fc特异性)-过氧化物酶 山羊抗

生物来源

goat

抗体形式

affinity isolated antibody

antibody product type

secondary antibodies

克隆

polyclonal

species reactivity

mouse

应用

direct ELISA: 1:50,000
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well

偶联物  peroxidase conjugate

运输  dry ice

储存温度  −20°C

 

 

一般描述

免疫球蛋白G(IgG)是一种糖蛋白抗体,其可调节免疫反应,如吞噬作用,也可参与自身免疫疾病的发展。小鼠IgG具有四种不同的同种型,即IgG1、IgG2a、IgG2b和IgG3。IgG1可调节小鼠的补体结合。
通过免疫特异性纯化从山羊抗小鼠IgG抗血清中分离抗体,该过程可基本上去除所有山羊血清蛋白,包括免疫球蛋白(其不可与小鼠IgG的Fc片段特异性结合)。以0.2%戊二醛为交联剂,将抗鼠IgG与过氧化物酶偶联。该抗体制剂采用人IgG吸附固相制备,以确保组织或细胞制剂中的交叉反应性最低。

免疫原

纯化的小鼠IgG Fc片段

应用

通过使用生物合成PND和链霉亲和素涂层板进行ELISA试验,从而对免疫小鼠血液中一种新的PND抗体的特异性进行检测。HRP缀合的山羊抗小鼠IgG(Fc特异性)可用作二抗。

山羊抗小鼠IgG (Fc特定)-过氧化物酶抗体用于:

  • 免迹印迹
  • 酶联免疫吸附测定(ELISA)和
  • 间接免疫过氧化物酶测定(IIPA)

 

外形

0.01M磷酸缓冲盐缓冲液(pH7.4, 含有0.05% MIT)溶液。

制备说明

通过Avrameas, S., et al., Scand.J. Immunol., 8, Suppl. 7, 7 (1978)等描述的两步戊二醛方法进行制备。

价格库存
PD1-H52A3 ACRO Human PD-L1 / B7-H1 Protein, Mouse IgG1 Fc Tag, low endotoxin (MALS verified)
Human PD-L1 / B7-H1 Protein, Mouse IgG1 Fc Tag, low endotoxin (MALS verified)

Human PD-L1 / B7-H1 Protein, Mouse IgG1 Fc Tag, low endotoxin (MALS verified)

分子别名(Synonym)

PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

表达区间及表达系统(Source)

Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (PD1-H52A3) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).

Predicted N-terminus: Phe 19

蛋白结构(Molecular Characterization)

Online(Phe 19 - Arg 238) NP_054862.1

This protein carries a mouse IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 51.4 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 24 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
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